Class II Tetramer Production
Tetramer reagents for more than 30 different HLA class II alleles are produced through the Tetramer Core Laboratory. These reagents can be used to study human CD4+ T cell responses over a wide cross section of the population. Class II tetramers that can be used for CYTOF mass cytometry are also being produced in the core laboratory.
T Cell Epitope Discovery
A robust, generalized approach has been developed and implemented to systematically identify CD4+ T cell epitopes. This approach is applied to identify T cell epitopes within Categories A, B and C pathogens, tumor antigens, allergens and antigens associated with autoimmune diseases in humans.
Tetramers and other antigen specific T cell assays are used to examine autoreactive T cells in type 1 diabetes, multiple sclerosis, systemic lupus erythematosus and other autoimmune diseases. Experiments are designed to determine the molecular mechanisms of HLA and autoimmune disease association. HLA that are linked to disease susceptibility or disease protection have been identified. Contrasting the behavior of T cells restricted by susceptible and protective HLA alleles should provide insights into disease mechanisms and suggest strategies for intervention.
Bill Kwok, PhD
Timothy Kountz, PhD
Allergen epitope specific CD4+ T cells
CD4+ and CD8+ T cells in Rheumatoid Arthritis
DNA-barcoded HLA class II tetramers
Using Your Own Cells to Fight Autoimmune Disease
For nearly 20 years, Dr. Buckner has been working to find a more targeted approach with fewer side effects. And she’s among a handful of scientists pursuing an intriguing idea: Can we use gene editing to change cells that cause disease into cells that protect you from disease?
The Power of Tetramers: A Tool Developed by Bill Kwok, PhD
Bill Kwok, PhD, has made groundbreaking discoveries and built a tool that’s fueled countless advances. But he makes his work and his path to BRI sound simple: Why study the minute details of the immune system? Well, he’s curious — he was always at the library as a kid.
BRI Races to Understand COVID-19
BRI scientists were sure of one thing when the novel coronavirus struck: They had the expertise to make swift progress toward understanding it and finding better treatments. “Studying how the immune system responds to viruses has been part of our work for years,” says BRI’s President Jane Buckner