Introduction
The Bolouri lab is interested in developing and applying computational systems immunology methods to better understand dysregulated immune cell development and differentiation.
Our approach is inherently multi- and cross-disciplinary. We integrate biological and immunological knowledge with data from high-throughput assays using machine-learning, neural networks and other Data Science methods derived from computer science, engineering, mathematics, statistics and physics.
To get a more complete picture of the system of interest, we typically integrate data from diverse assays (e.g. single-cell RNAseq, CITE-seq, single-cell ATAC-seq, flow and mass cytometry, etc.) with patient clinical data and curated data from databases and publications.
Associate Member
Hamid Bolouri, PhD
Associate Member, Center for Systems Immunology; Principal Investigator, Bolouri Lab; Affiliate Faculty, Division of Human Biology, Fred Hutchinson Cancer Research Center
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Research Projects
Bias-aware data integration
We correct for known assay-specific biases before multi-modal data integration.
Inflammatory signaling in pediatric Acute Myeloid Leukemia
Inflammatory signaling dysregulates the development of myeloid cells in the bone marrow and marks resistance to chemotherapy.
Regulation of blood homeostasis
What are the regulatory mechanisms that maintain highly stable immune cell frequencies in the blood?
Featured Publications
Dec 2023
Altered Treg Infiltration after Discoidin Domain Receptor 1 (DDR1) Inhibition and Knockout Promotes Tumor Growth in Lung Adenocarcinoma.
Cancers (Basel)
Maitz K, Valadez-Cosmes P, Raftopoulou S, Kindler O, Kienzl M, Bolouri H, Houghton AM, Schicho R, Heinemann A, Kargl J
Nov 2022
Inflammatory bone marrow signaling in pediatric acute myeloid leukemia distinguishes patients with poor outcomes.
Nat Commun
Bolouri H, Ries RE, Wiedeman AE, Hylkema T, Scheiding S, Gersuk VH, O'Brien K, Nguyen QA, Smith JL, Long SA, Meshinchi S
Oct 2022
Oncogenic role of a developmentally regulated NTRK2 splice variant.
Sci Adv
Pattwell SS, Arora S, Nuechterlein N, Zager M, Loeb KR, Cimino PJ, Holland NC, Reche-Ley N, Bolouri H, Almiron Bonnin DA, Szulzewsky F, Phadnis VV, Ozawa T, Wagner MJ, Haffner MC, Cao J, Shendure J, Holland EC
Nov 2021
A B-cell developmental gene regulatory network is activated in infant AML.
PLoS One
Bolouri H, Ries R, Pardo L, Hylkema T, Zhou W, Smith JL, Leonti A, Loken M, Farrar JE, Triche TJ Jr, Meshinchi S
Apr 2021
Multimodal analysis for human ex vivo studies shows extensive molecular changes from delays in blood processing.
iScience
Savage AK, Gutschow MV, Chiang T, Henderson K, Green R, Chaudhari M, Swanson E, Heubeck AT, Kondza N, Burley KC, Genge PC, Lord C, Smith T, Thomson Z, Beaubien A, Johnson E, Goldy J, Bolouri H, Buckner JH, Meijer P, Coffey EM, Skene PJ, Torgerson TR, Li XJ, Bumol TF