Tetramer Core Laboratory
Tetramers produced in our facility have been used to detect CD4+ (helper) T cells and have facilitated the study of viral immunity, autoimmune diseases, allergic responses and cancer. The class II MHC proteins are produced using stable insect cell cultures and purified by affinity chromatography. The purified proteins are loaded with exogenous peptide and conjugated using PE labeled streptavidin. In some cases we are able to provide T cell clones for use as experimental positive controls.
Our intention is to distribute tetramer reagents as widely as possible. To receive materials from the Tetramer Core, users must complete a material transfer agreement. Unless alternative arrangements are made, all users must provide peptide solution (20-50 mg/mL in DMSO) for each tetramer to be delivered.
DRB1*01:01, DRB1*01:02, DRB1*03:01, DRB1*04:01, DRB1*04:02, DRB1*04:03, DRB1*04:04, DRB1*04:05, DRB1*07:01, DRB1*09:01, DRB1*10:01, DRB1*11:01, DRB1*11:04, DRB1*12:01, DRB1*13:01, DRB1*13:02, DRB1*14:01, DRB1*15:01, DRB3*01:01, DRB3*02:02, DRB4*01:01, DRB5*01:01, DPB1*04:01, DPB1*05:01, and DQB1*06:02
For more information
Differentiation stage determines pathologic and protective allergen-specific CD4(+) T-cell outcomes during specific immunotherapy. Wambre E, Delong JH, James EA, Lafond RE, Robinson D, Kwok WW. J Allergy Clin Immunol. 2011 Oct 3. [Epub ahead of print]
Human CD8+ and CD4+ T Cell Memory to Lymphocytic Choriomeningitis Virus Infection. Kotturi MF, Swann JA, Peters B, Arlehamn CL, Sidney J, Kolla RV, James EA, Akondy RS, Ahmed R, Kwok WW, Buchmeier MJ, Sette A. J Virol. 2011 Nov;85(22):11770-80. Epub 2011 Sep 7.
Uveitis-associated epitopes of retinal antigens are pathogenic in the humanized mouse model of uveitis and identify autoaggressive T cells. Mattapallil MJ, Silver PB, Mattapallil JJ, Horai R, Karabekian Z, McDowell JH, Chan CC, James EA, Kwok WW, Sen HN, Nussenblatt RB, David CS, Caspi RR. J Immunol. 2011 Aug 15;187(4):1977-85. Epub 2011 Jul 15.
Papillomavirus-specific CD4+ T cells exhibit reduced STAT-5 signaling and altered cytokine profiles in patients with recurrent respiratory papillomatosis. James EA, DeVoti JA, Rosenthal DW, Hatam LJ, Steinberg BM, Abramson AL, Kwok WW, Bonagura VR. J Immunol. 2011 Jun 1;186(11):6633-40. Epub 2011 Apr 29.
Ara h 1-reactive T cells in individuals with peanut allergy. DeLong JH, Simpson KH, Wambre E, James EA, Robinson D, Kwok WW. J Allergy Clin Immunol. 2011 May;127(5):1211-8.e3. Epub 2011 Apr 2.
T-cell responses in two unrelated hemophilia A inhibitor subjects include an epitope at the factor VIII R593C missense site. James EA, van Haren SD, Ettinger RA, Fijnvandraat K, Liberman JA, Kwok WW, Voorberg J, Pratt KP. J Thromb Haemost. 2011 Apr;9(4):689-99. doi: 10.1111/j.1538-7836.2011.04202.x.
Chow IT, James EA, Tan V, Moustakas AK, Papadopoulos GK, Kwok WW. DRB1*12:01 presents a unique subset of epitopes by preferring aromatics in pocket 9. Mol Immunol. 2012 Feb;50(1-2):26-34. doi: 10.1016/j.molimm.2011.11.014. PMC3433834
Kwok WW, Tan V, Gillette L, Littell CT, Soltis MA, LaFond RB, Yang J, James EA, DeLong JH. Frequency of epitope specific naïve CD4+ T cells correlates with immunodominance in the human memory repertoire. J Immunol. 2012 Mar 15;188(6):2537-44. PMID:22327072
Arlehamn CS, Sidney J, Henderson R, Greenbaum JA, James EA, Moutaftsi M, Coler R, McKinney DM, Park D, Taplitz R, Kwok WW, Grey H, Peters B, Sette A. Dissecting mechanisms of immunodominance to the common Tuberculosis antigens ESAT-6, CFP10, Rv2031c (hspX), Rv2654c (TB7.7), and Rv1038c (EsxJ). J Immunol. 2012 May 15;188(10):5020-31. PMC3345088
Yang J, James EA, Sanda S, Greenbaum C, Kwok WW. CD4+ T cells recognize diverse epitopes within GAD65: implications for repertoire development and diabetes monitoring. Immunology 2013 Mar;138(3):269-79. doi: 10.1111/imm.12034. PMC3573280
Lindestam Arlehamn CS, Gerasimova A, Mele F, Henderson R, Swann J, Greenbaum JA, Kim Y, Sidney J, James EA, Taplitz R, McKinney DM, Kwok WW, Grey H, Sallusto F, Peters B, Sette A. Memory T cells in latent Mycobacterium tuberculosis infection are directed against three antigenic islands and largely contained in a CXCR3(+)CCR6(+) Th1 subset. PLoS Pathog. 2013 Jan;9(1):e1003130. doi: 10.1371/journal.ppat.1003130. PMC3554618
Chow I-T, James EA, Gates TJ, Tan V, Moustakas AK, Papadopoulos GK, Kwok WW. Differential binding of pyruvate dehydrogenase complex-E2 epitopes by DRB1*08:01 and DRB1*11:01 is predicted by their structural motifs and correlates with disease risk. J Immunol. 2013 May 1;190(9):4516-24. doi: 10.4049/jimmunol.1202445. PMID: 23543758
Yang J, James E, Gates TJ, DeLong JH, LaFond RE, Malhotra U, Kwok WW. CD4+ T cell recognize unique and conserved 2009 H1N1 hemagglutinin epitopes after natural infection and vaccination. Inter Immunol. 2013 Mar 22. PMID: 23524391