Michael G. Kinsella, PhD
Research Associate Member
Dr. Kinsella attended the University of Washington, from which he received his Bachelor of Science degree in biology. His received his PhD in developmental neurobiology in 1978 from the University of Oregon. After postdoctoral work in heart developmental mechanisms and as an Instructor of Histology and Embryology in the Department of Anatomy and Cell Biology at the Medical University of South Carolina, he returned to Seattle, joining the University of Washington Department of Pathology Faculty in 1987, where he remains an Affiliate Associate Professor. Since 2005, he has been a Research Associate Member within the Hope Heart Matrix Biology Program of the Benaroya Research Institute at Virginia Mason.
Areas of Research
Dr. Kinsella leads research efforts to understand how the extracellular matrix within tissue regulates vascular and immune cell responses during development, injury, inflammation and disease. His laboratory has extensive experience in analysis and chemical modification of heparin/heparan sulfates, which have been used in in vitro and in vivo models of cell behavior and experimental disease. In addition, since extracellular matrix components such as versican and hyaluronan are synthesized during acute and chronic inflammatory diseases, the team has designed molecular tools, including retrovirally-mediated expression in vivo and in vitro as well as transgenic and knock-out animal models, to examine the roles of these molecules during vascular injury and in experimental models of lung inflammation. The lab studies of the regulation of vascular tissue structure has recently led to the development of recombinant protein expression systems for production of versican-related proteins that may allow scientists to regulate the structure, resiliency and patency of engineered vessel replacements.
Kinsella MG, Fischer JW, Mason DP and Wight TN. Retrovirally-mediated expression of decorin by macrovascular endothelial cells: effects on cellular migration and fibronectin fibrillogenesis in vitro. J Biol Chem 275:13924-13932, 2000.
Merrilees MJ, Lemire JM, Fischer JW, Kinsella MG, Braun KR, Clowes AW, and Wight TN. Retrovirally mediated overexpression of versican V3 by arterial smooth muscle cells induces tropoelastin synthesis and elastic fiber formation in vitro and in neointima after vascular injury. Circ Res 90:481-487, 2002.
Kinsella MG, Tran PK, Weiser-Evans MC, Reidy M, Majack RA and Wight TN. Changes in perlecan expression during vascular injury: role in the inhibition of smooth muscle cell proliferation in the late lesion. Arterioscler Thromb Vasc Biol 23:608-614, 2003.
Frevert CW, Kinsella MG, Vathanaprida C, Goodman RB, Baskin DG, Proudfoot A, Wells TN, Wight TN, and Martin TR. Binding of interleukin-8 to heparan sulfate and chondroitin sulfate in lung tissue. Am J Respir Cell Mol Biol 28:464-472, 2003.
Sakr SW, Potter-Perigo S, Kinsella MG, Johnson PY, Braun KR, Gouëffic Y, Rosenfeld ME, and Wight TN. Hyaluronan accumulation is elevated in cultures of LDL receptor-deficient cells and is altered by manipulation of cell cholesterol content. J Biol Chem 283:36195-36204, 2008.
Cardoso LEM, Little PJ, Ballinger ML, Chan CK, Braun KR, Potter-Perigo S, Bornfeldt KE, Kinsella MG, and Wight TN. Platelet-derived growth factor differentially regulates the expression and post-translational modification of versican by arterial smooth muscle cells through distinct protein kinase C and extracellular signal-regulated kinase pathways. J Biol Chem 285:6987-95, 2010.
Braun KR, DeWispelaere AM, Bressler SL, Fukai N, Kenagy RD, Chen L, Clowes AW, and Kinsella MG. Inhibition of PDGF-B induction and cell growth by syndecan-1 involves the ubiquitin and SUMO-1 ligase, Topors. PLoS One 7:e43701, 2012.
Hull RL, Peters MJ, Potter-Perigo S, Chan CK, Wight TN, and Kinsella MG. Overall sulfation of heparan sulfate from pancreatic islet β-TC3 cells increases maximal fibril formation but does not determine binding to the amyloidogenic peptide islet amyloid polypeptide. J Biol Chem 287:37154-37164, 2012.