"Aru" K. Arumuganathan, PhD
Director, Flow Cytometry Core Lab and Research Associate Member
Dr. Arumuganathan received his bachelor’s degree from Sri Lanka and Master’s degree from University of Wales, UK. He attended Ohio University, receiving his PhD in Molecular and Cell Biology in 1988. After post-doctoral work in chromosome sorting at Cornell University in Ithaca, NY, he joined the University of Nebraska Faculty in 1992. Since 2001, he has been Research Associate Member and Director of the Flow Cytometry Core Laboratory at Benaroya Research Institute at Virginia Mason.
Areas of Research
As the primary source and contact for flow cytometric services, Dr Arumuganathan ensures successful operation of the BRI Flow Cytometry Core Laboratory. This includes sorting human and murine specimens for BRI’s research projects, providing technical support and training for flow cytometry users, and maintaining facility instrumentation: a 3-laser BD FACS Diva high-speed sorter, a 3-laser BD LSRII, a three dual laser BD FACSCaliburs, and a single laser BD FACSort.
Dr. Arumuganathan has an expertise in isolation, flow karyotyping and sorting of chromosomes. His current collaborative research activities include flow cytometric estimation of genome sizes, sorting of mitochondria and chloroplast.
Arumuganathan K,Slattery JP, Tanksley SD, Earle ED. (1991). Preparation and flow cytometric analysis of metaphase chromosomes of tomato. Theoretical and Applied Genetics 82: 101-111.
Arumuganathan K, Earle ED. (1991). Estimation of nuclear DNA amounts of plants by flow cytometry. Plant Molecular Biology Reporter 9 (3): 229-241
Arumuganathan K, Earle ED. (1991). Nuclear DNA content of some important plant species. Plant Molecular Biology Reporter 9 (3): 208-219.
Arumuganathan K, Martin GB, Telenius H, Tanksley SD, Earle ED (1994). Chromosome 2-specific DNA clones from sorted chromosomes of tomato. Molecular and General Genetics 242: 551-558.
Lee JH, Arumuganathan K, Kaeppler SM, Kaeppler HF, Papa CM. (1996). Cell synchronization and isolation of metaphase chromosomes from maize root tips for flow-cytometric analysis and sorting. Genome 39: 697-703.
Lee JH, Arumuganathan K,Yen Y, Kaeppler SM, Kaeppler HF, Baenziger PS. (1997). Root tip cell cycle synchronization and metaphase chromosomes isolation suitable for flow sorting in common wheat (Triticum aestivum L.) Genome 40: 633-638.
Gill KS, Arumuganathan K, Lee J.-H. (1999). Isolating individual wheat (Triticum aestivum) chromosome arms by flow cytometric analysis of ditelosomic lines. Theor. Appl. Genet. 98 (8): 1248-1252.
Li L, Arumuganathan K, Rines HW, Phillips R, Riera-Lizarazu O, Sandhu D, Zhou Y, Gill KS. (2001). Isolating maize chromosome 9 from oat-maize chromosome addition lines by flow cytometric sorting. Theor. Appl. Genet. 102 (5): 658-663.
Wang, W., M. Tanurdzic, M. Luo, N. Sisneros, H.R. Kim, J.K. Weng, D. Kudrna, C. Mueller, K. Arumuganathan, J. Carlson, C. Chapple, C. de Pamphilis, D. Mandoli, J. Tomkins, R.A. Wing, and J.A. Banks. 2005. Construction of a bacterial artificial chromosome library from the spikemoss Selaginella moellendorffii: a new resource for plant comparative genomics. BMC Plant Biol 5:10.
Tuna M, Vogel KP, Arumuganathan K. (2005) Genome size and Giemsa C-banded karyotype of tetraploid Bromus ciliatus L. Euphytica 146: 177-182.
Cui L, Wall PK, Leebens-Mack JH, Lindsay BG, Soltis D, Doyle JJ, Soltis P, Carlson JE, Arumuganathan K, Barakat A, Albert V, Ma H, dePamphilis CW. 2006. Widespread genome duplications throughout the history of flowering plants. Genome Research 16: 738 - 749.
Ammiraju JSS, Luo M, Goicoechea JL, Wang W, Kudrna D, Mueller C, Talag J Kim H, Sisneros NB, Blackmon B, Fang E, Tomkins JB, Brar D, MacKill D, McCouch S, Kurata N, Lambert G, Galbraith DW, Arumuganathan K, Rao K, Walling JG, Gill N, Yu Y, SanMiguel P, Soderlund C, Jackson S, Wing RA. (2006). The Oryza bacterial artificial chromosome library resource: construction and analysis of 12 deep-coverage large-insert BAC libraries that represent the 10 genome types of the genus Oryza Genome Res. 16(1):140-147.
McNeal JR, Arumugunathan K, Kuehl JV, Boore JL, dePamphilis CW. 2007 Systematics and plastid genome evolution of the cryptically photosynthetic parasitic plant genus Cuscuta (Convolvulaceae). BMC Biology, 5: 55-64.